Dr. Johannes Härle

Angestellt, Research Scientist, Evolva Holding SA

Basel, Schweiz

Fähigkeiten und Kenntnisse

Cellular Biology and Biochemistry including
PCR-techniques
vector manipulation
in situ gene recombination
heterologous gene expression
strain engineering technologies
Western-blot
Southern-blot
Northern-blot
ELISA
autoradiography
DNA and protein gel electrophoresis techniques
protein engineering
in silico protein design
recombinant protein expression and purification
operating Äkta FPLC systems
GPC
IEC
IMAC
HPLC-ESI-MS
HPLC-APCI-MS
MS/MS
real time MS
FACS analysis
laboratory automation systems
UV/Vis-Spektroskopy
Luminex Bead Systems
Panomics Hybridisation Systems

Werdegang

Berufserfahrung von Johannes Härle

  • Bis heute 9 Jahre und 3 Monate, seit März 2015

    Research Scientist

    Evolva Holding SA

  • 3 Jahre und 2 Monate, Sep. 2011 - Okt. 2014

    Postdoc

    ETH Zurich, Dept. of Biosystems Science and Engineering, Bioprocess Laboratory

  • 3 Jahre und 3 Monate, Apr. 2008 - Juni 2011

    PhD student

    University Freiburg, Pharmaceutical Sciences, Dept. of Pharmaceutical Biology

    PhD thesis “Rational design of a C-glycosyltransferase & MazF toxin as a molecular biological tool in Streptomyces”

  • 9 Monate, Mai 2007 - Jan. 2008

    Diploma student

    University Tübingen, Pharmaceutical Sciences, Dept. of Pharmaceutical Biology

    Diploma thesis “Investigation of regulatory mechanisms of the novobiocin biosynthetic gene cluster in Streptomyces spheroides”

  • 3 Monate, Jan. 2007 - März 2007

    Research assistant

    Scripps Institution of Oceanography

    Engineering studies on a bacterial halogenase by rational protein design, biochemical characterisation of DUF 62 (Domain with unknown funktion), an enzyme with SAM-hydrolase activity.

  • 6 Monate, Aug. 2006 - Jan. 2007

    Research assistant

    Naturwissenschaftliches und Medizinisches Institut Reutlingen

    Successfully performed transglutaminase catalyzed coupling reactions for constructing matrices for artificial tissue culturing. Practical knowledge in FPLC analysis and purification methods. Operation experience in performing fluorescent microscopy based cell migration assays.

  • 6 Monate, Aug. 2005 - Jan. 2006

    Research assistant

    University Tübingen, Pharmaceutical Sciences, Dept. of Pharmaceutical Biology

    Experience in cultivating and isolating secondary metabolites from genetically modified fungi. Quantitation of the bioactive natural product fumgaclavin from Penicillium commune by HPLC analysis.

  • 2 Jahre und 5 Monate, März 2003 - Juli 2005

    Research assistant

    University Tübingen,Center for Plant Molecular Biology

    Successfully gene insertion experiments in Arabidopsis thaliana with the Agrobacterium Ti-plasmid. Experience in performing plant stress response experiments by investigating cell necrosis and cell apoptosis with light microscopy.

  • 11 Monate, Aug. 2000 - Juni 2001

    Research assistant, civilian service

    University Hospital Tübingen, Institute of Medical Virology and Epidemiology

    Processing human samples and diagnosis of human virus infections. Human cell culturing for daily investigations.

Ausbildung von Johannes Härle

  • 1 Jahr und 6 Monate, Aug. 2016 - Jan. 2018

    EMBA

    University of Zurich

  • 3 Jahre und 3 Monate, Apr. 2008 - Juni 2011

    Pharmacy

    University Freiburg

    Pharmaceutical Biology and Biotechnology

  • 5 Jahre und 4 Monate, Okt. 2002 - Jan. 2008

    Biochemistry

    University Tübingen

    Biochemistry, Pharmaceutical Chemistry, Pharmaceutical Biology

Sprachen

  • Deutsch

    -

  • Englisch

    -

Interessen

Life sciences
outdoor sports
wind instruments

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