Meenakshi Anil
Angestellt, Senior Research Associate, Syngene International Ltd
Bengaluru, Indien
Über mich
Science enthusiast | Specialized in Protein Biochemistry | Experienced in purification and analysis of proteins (pilot scale and large scale) | Proficient in cell culture and expression systems (mammalian, bacterial and insect) | Skilled in protein cleavage processes such as TEV and caspase reactions | Well versed with different protein separation and processing approaches including protein fractionation, various chromatographic techniques (affinity, SEC, IEX, HIC, etc), filteration methods (TFF - UF/DF, etc) as well as dialysis | Adept in developing efficient strategies for the best analysis of different protein variants employing SEC HPLC (Agilent) | Expert in AKTA and Biorad Duo flow FPLC Systems
Werdegang
Berufserfahrung von Meenakshi Anil
Bis heute 3 Jahre und 10 Monate, seit Aug. 2020
Senior Research Associate
Syngene International Ltd
- Purification and analysis of proteins (pilot and large scale): mAbs, Fabs, scfvs, ILs, enzymes, growth factors, bispecifics, trispecifics, recombinant proteins - Optimization of protein cleavage and modification processes such as TEV and caspase reactions, biotinylation, deglycosylation - Standardization and method development for efficient analysis of different protein molecules employing SEC HPLC (Agilent) - ALCOA+ - CAPA - Standardization of endotoxin removal from protein samples -
1 Jahr und 8 Monate, Aug. 2018 - März 2020
Junior Research Fellow
Amrita Institute of Medical Sciences and Research Center
-Baculovirus mediated expression, purification and characterization of humanized anti-EGFRvIII scfv in SF21 insect cells - Mentored and trained masters students. -Assisted “Study on the expression of pathologically relevant proteins involved in Diabetic Retinopathy in astrocytes and muller cells” (Novartis) - compared and evaluated the effect of aqueous and vitreous humor from patients with Diabetic Retinopathy on vital pathologically relevant molecules in astrocytes and muller cells.
1 Jahr und 3 Monate, Mai 2017 - Juli 2018
Master's Dessertation - Research Trainee
Amrita Institute of Medical Sciences and Research Center
- Evaluated the effect of CSF from patients (MS and other neurological disorders) on astrocytic molecules (GMF b, QDPR, PHB1, DJ1, GM-CSF, iNOS, HMGB1, NO) - Studied the influence of QDPR and GMF b on the immunologically active molecules in astrocytes -Accomplished tranfection, enhanced expression and purification of QDPR and GMF b in astrocytes (CTX.TNA2) -Performed antibody purification from MS patients CSF samples and studied the reactivity of the purified antibody fraction towards QDPR.