Dr. Praneeth Chitirala

is researching.

Abschluss: PhD, Universität des Saarlandes

Homburg, Deutschland

Fähigkeiten und Kenntnisse

Cloning
siRNA and shRNA designing and knockdown in cell li
Immunoprecipitations of tagged proteins
Generation of knock-in mice
Southern blotting
Confocal microscopy (ZEISS LSM 710)
Structured Illumination microscopy (SIM)
Total Internal Reflection Microscopy (TIRFM)
Culturing of primary human and mouse CD8+ T cells
Bacterial transformation
Fixing cells and immunostaining
Protein expression
Protein purification
Pulldown assays
Western Blotting
Membrane fractionation
Polyclonal antibody generation in rabbits
Killing assay and degranulation assay in primary T
Di- and Trihybrid assays and transformation in yea
SnapGene
ImageJ (Fiji)
SigmaPlot
CorelDraw
Affinity Designer

Werdegang

Berufserfahrung von Praneeth Chitirala

  • Bis heute 4 Jahre und 5 Monate, seit Jan. 2020

    Postdoctoral Researcher

    Uniklinik Homburg

  • 3 Jahre und 3 Monate, Jan. 2011 - März 2014

    Research Associate

    Indian Institute of Science

    I was the first Research Associate in the lab of Dr. Subba Rao, Microbiology Department, Indian Institute of Science, Bangalore, India. I established and standardised many techniques in the lab. Worked on different projects and my main project was entitled as ''ARL4A, an ARF-family GTPase regulates endosomal transport to melanosome''.

Ausbildung von Praneeth Chitirala

  • 5 Jahre und 9 Monate, Apr. 2014 - Dez. 2019

    Molekulare Zellbiologie und Immunologie

    Universität des Saarlandes

    Immunology, Molecular Biology and Protein trafficking

Sprachen

  • Englisch

    Fließend

  • Deutsch

    Grundlagen

Interessen

Badminton (player of Homburg Badminton team)
Running

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