Dr. Sergii Krysenko

- Strain Engineering: design and build new strains to optimize production of key metabolites and proteins. - Establish genetic tractability in novel strains of fungal and bacterial origin. - Mentor research associates in Synthetic Biology to assure team success. - Design and build a set of in-house molecular tools to facilitate engineering model and non-model organisms including yeast, bacteria and fungi.

- Management of a research group: supervision of students and technical assistants, project management: reports, meetings, cooperations with academia and industry, networking, interaction with funding agencies, drug development, protein biochemistry, computational biology, down- and up-stream processing Web: https://uni-tuebingen.de/fakultaeten/mathematisch-naturwissenschaftliche-fakultaet/fachbereiche/interfakultaere-einrichtungen/imit/arbeitsgruppen/mikrobiologie-biotechnologie/research-groups/krysenko/

- Biochemistry: protein production, design, engineering and purification, FPLC/ÄKTA (AC, SEC, IEX), in vitro assays for protein activity, substrate specificity, inhibitor tests etc. - Analytics: HPLC, LC-MS, MS/MS - Computational biology: PyMOL, Chimera, SwissModel/SwissPDBviewer, AutodockVina, Biopython - Drug development: drug design, target validation, target-drug interaction, synthetic biology - Working under GSP / GMP guidelines

- Microbiology/biotechnology: upstream processing, cultivation/fermentation of different industrially relevant bacteria, secondary metabolite production and isolation, media development, phenotypical analysis - Molecular biology: genetic engineering, molecular cloning, vector and primer design, knock-out /-in mutant generation, site-directed mutagenesis, conjugation with different hosts, Southern- /Western-blot, sequencing - Bioinformatics: protein modelling, sequence analysis, data analysis / visualization

- Molecular biology: genetic engineering, molecular cloning, vector and primer design, knock-out, knock-in mutant generation, conjugation with different hosts, Southern-blot, sequencing - Bioinformatics: sequence analysis, phylogeny, data analysis and visualization, data mining

Internship (certified) / volunteer practice to gain experience in IT along with studying biology: - administration of computers in the Linux environment (mostly Red Hat distribution) - administration of Linux based network - preparation of technical documentation about Linux usage (e.g. about WINE) - user support

Internship (certified) / freelance practice to gain experience in IT and data analysis along with studying biology: - analysis of cartographic data in AutoCAD - preparation of technical documentation using AutoCAD - evaluation of cartographic data in ArcGIS - support with web-site maintenance (HTML/CSS) - support with UNIX-based server maintenance (FreeBSD, RedHat Linux)

Internship (certified) / volunteer practice to gain experience in data analysis in the university´s department for public affairs: - data research and analysis using MS Excel, - working with databases, - coordination of students.