Ulrike Ritter

High throughput transcriptomics

High throughput transcriptome analysis - Sample preparation for NGS, starting from RNA extraction to ready-to-sequence libraries - Handling various sample types, e.g. cells or samples with animal origin like blood - Protocol automation with various liquid handling systems - Continuous protocol optimization & establishment of new assays - Proactive role in improving processes for the planning phase of new projects, the lab work & documentation Labmangement - coordination, organization, device management

Transcriptomics Next Generation Sequencing Automation Labmanagement

Analysis of the expression level of ubiquitin E3 ligases due to bacterial and viral mono-infection - Fundamental techniques of sterile cell cultivation, microbiology and virology - Establishment of in vitro bacterial and viral mono-infection with S. pneumoniae and influenza A virus, resp. - Analysis of the expression level of ubiquitin E3 ligases by molecular biology and protein biochemistry-based techniques - Cell viability assays

Development of saliva-based drug tests - Development and optimization of screening tests to determinate illegal substances in human saliva - Validation of new immunchromatographic test systems for performance, precision and sensitivity

Functional characterization of the ubiquitin proteasom system in human chemoresistant tumor cell lines - Cultivation of human sarcoma cell lines - Western Blot analysis of the functional proteasome subunits expression - Activity analysis of individual subunits after inhibition - Analysis of NFκB signaling pathway

Clinical cooperation project ‘Applied Tumor biology’ (Universitätsklinikum Heidelberg/DKFZ) - Tumor biology of HPV-induced cancer - Analysis of HLA II- and p16INK4a-Expression in cervical cancer - immune histochemical staining, statistical evaluation of the expression patterns