Dr. Brinda Prasad

Angestellt, Post-doctoral researcher, Technische Universität Hamburg Harburg

hamburg, Deutschland

Fähigkeiten und Kenntnisse

a) Microbiology and biochemistry: Enzyme assays de
protein expression and purification
spectroscopic assays
enzyme purification and kinetics
b) Molecular biology: Primer design
Polymerase Chain Reaction
DNA and plasmid isolation
competent cell preparation
cloning
mutant library generation and screening assays
c) Organic Chemistry: NMR spectroscopy (1H
13C
17O)
TLC
HPLC
GC-MS

Werdegang

Berufserfahrung von Brinda Prasad

  • Bis heute 10 Jahre und 1 Monat, seit Juni 2014

    Post-doctoral researcher

    Technische Universität Hamburg Harburg

  • 5 Jahre und 4 Monate, Jan. 2008 - Apr. 2013

    Graduate Research assistant

    Simon Fraser University

    Elucidation of reduction mechanism for the enzyme cytochrome P450cam. - Involved NMR (proton, dueterium and 17O) to prove the reduction mechanism in borneol cycle. - Enriched H217O (natural abundance: 0.03%) from tap water by fractional distillation. - Preparation of mutant P450cam library by Sequence Saturation Mutagenesis (SeSaM) and screening of the library for enzyme assays.

  • 4 Jahre und 4 Monate, Mai 2008 - Aug. 2012

    Graduate Teaching assistant

    Simon Fraser University

    Lectured second-year undergraduate courses in organic chemistry - Assisted in the preparation and evaluation of exams

  • 9 Monate, Apr. 2007 - Dez. 2007

    Lecturer

    Sri Krishna Chaitanya College of Pharmacy

    Lectured undergraduate biochemistry courses - Handled chemistry and biochemistry laboratory courses for pharmacy students

  • 9 Monate, Juli 2006 - März 2007

    Research associate

    Lupin Pharmaceuticals

    Synthesized the polymorphs of antibiotic cefdinir varying the physiological conditions - Synthesized an intermediate ‘GCLE’ from Penicillin GK which was a key precursor for many cephalosporin derivatives - Performed large scale reactions in a pilot lab and handled patent issues of antibiotics in a pharmaceutical industry

Ausbildung von Brinda Prasad

  • 5 Jahre und 4 Monate, Jan. 2008 - Apr. 2013

    Ph.D.

    Simon Fraser University

    Chemistry, Biochemistry, Microbiology and Molecular biology

  • 3 Monate, Apr. 2005 - Juni 2005

    Chemistry

    Indian Institute of Technology

    Inorganic chemistry, Spectroscopy

  • 1 Jahr und 11 Monate, Juni 2004 - Apr. 2006

    Chemistry

    University of Pune

    Spectroscopy, Kinetics, Biochemistry, Organic synthesis

  • 2 Jahre und 11 Monate, Aug. 2001 - Juni 2004

    Biotechnology

    Osmania University

    Biotechnology, Biochemistry and Chemistry

Sprachen

  • Englisch

    Fließend

  • Deutsch

    Gut

  • Hindi

    -

  • Tamil

    -

  • Telugu

    -

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