Lisa Pitzschler

student assistant on research vessel Polarstern - experimental setup - respirations measurements - data analysis - filtration (CTD)

-microscopy -sample preparation -RNA extraction

Master Thesis: „Daily patterns of clock gene expression in Antarctic krill, E. superba, under a 12h:12h light:dark cycle in the laboratory“ (1.6) Methods: - microscopy - RNA extractions - RNA purifications - RNA quantification - cDNA synthesis - Primer design - TaqMan RT-qPCR - data analysis

research project: „Circadian rhythms in respiration rates of the Antarctic krill E. superba” (1.0) - experimental setup - respiration measurements - data analysis - sample collection for RNA extraction

- tissue extraction for analysis of the metabolome 1H-NMR measurements (NMR- spectroscopy) - sample preparation for HPLC - cDNA synthesis - RNA extraction and RNA quality assessment

Bachelor thesis: Impact of natural killer cell (NK)-derived interferon-γ on viral clearance (1.3) (Immunohistochemistry and flow cytometry), molecular biological methods (PCR and agarose gel electrophoresis) - work with laboratory animals - infection experiments with vaccinia virus - immunohistochemical staining - flow cytometry - PCR analysis - agarose gel electrophoresis - cell culture

Molekularbiologie (Methoden: RNA Isolation und Quantifikation, cDNA Synthese, Primer-Design und RT-qPCR), Mikrobiologie, Analytik (Methoden: NMR-Spektroskopie, HPLC), Meereschemie, Gewässerschutz

Molekularbiologie (Methoden: PCR und Agarosegelelektrophorese), Analytik (Methoden: MS, IR-Spektroskopie, HPLC), Immunologie (Methoden: Immunhistochemie und Durchflusszytometrie) Virologie, Zellkultur