Dipl.-Ing. Marcel Thiele

- Sales Representative for genomics, cell-based assays, reportergen analysis etc. - Providing training seminars for customers in the field of qRT-PCR workflow - Product demonstrations - Representation at exhibitions, e.g. Biotechnica - Marketing support in terms of designing flyers/brochures, planning special promotions, developing and publishing of customer protocols - Taking care of Promega´s on-site stocking program (Helix®) - Area of work: Berlin, Potsdam and northeast of Germany

- Diploma thesis: ‘Functional analysis of the mediator protein Med12 during endoderm development in mice’. Grade: 1.0 - Tutorial leader in practical courses for medical students - Mouse husbandry - Histology - In situ hybridization (whole-mount and slides) - Immunohistochemistry and Immunofluorescence (whole-mount and slides) - TUNEL assay (whole-mount and slides) - 3D intestinal organoid culture - ES cell culture techniques - Basic molecular biology techniques

- Student Research Project thesis: ‘Optimization of a contact-free and robust spotting process in nanoliter range using the example of microarrays’. Grade: 1.0 - Microscopy: Scanning Electron Microscopy (SEM), Laser Scanning Microscopy (LSM), Transmitted Light Microscopy - Production of microarrays, analysis with biochip reader (Tecan LS Reloaded 200) - Determination of spot volume in nanoliter range - Basic cell culture techniques (adherent and suspension cells)

- DNA extraction, purification, and quantification - PCR (Polymerase Chain Reaction) - Silver staining of denaturing gradient gel electrophoresis (DGGE) gels - ELISA (Enzyme-Linked Immunosorbent Assay) - Measurement of nitric oxide via Griess reaction - Gram staining - FISH (Fluorescence in situ Hybridization)

- Preparation of mouse tissues - Phenol-chloroform extraction of genomic DNA - PCR (Polymerase chain reaction) - Cultivation of mouse embryonic fibroblasts - SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) - Western blots - Extraction of recombinant DNA polymerase - Purification of GST (glutathione-S-transferase) fusion proteins via affinity chromatography - RT-PCR (Reverse transcription Polymerase Chain Reaction)