Dr. Marsilius Mues

Interdisciplinary research and development in the field of immuno-oncology and cellular therapeutics, with a focus on CAR T cell engineering for personalized immunotherapy. Project management tasks to develop and establish multiparametric immune assays for functional characterization of engineered T cells, along with automated data acquisition and analysis tools for translational research, cell therapy manufacturing, and patient immune monitoring during clincial trials.

Interdisciplinary research and development in the field of immuno-oncology, with a focus on CAR T cell engineering for personalized immunotherapy. Project management tasks to establish multiparametric immune assays, functional characterization of cellular therapeutics, and automated data acquisition and analysis tools for translational research, in-process control of cell manufacturing, and patient immune monitoring during clincial trials.

Focus on understanding how different biochemical pathways cooperate in an aberrant manner in T cell leukemia with the goal of interfering with these leukemogenic signals. Analysis of oncogenic driver mutations in bone marrow chimeras and screening for novel combinatorial therapies using high-complexity lentiviral shRNA libraries. http://rooselab.ucsf.edu

Optimization of fluorescent reporters for in vivo analysis of signaling patterns in neuronal networks and auto-reactive T lymphocytes.

Characterization of calcium signaling pathways in autoreactive T cells using genetically encoded calcium indicators. Extensive optimization of retroviral transduction and transgene expression in primary T cells, which for the first time allowed direct in vivo observation of the complex activation patterns in immune cells during disease unfolding.

Functional analysis of the protective role of N-glycosylation and domain architecture of variant surface glycoproteins of the blood parasite Trypanosoma brucei, the causing agent of sleeping sickness.

Studies on the role of the mammalian RNA polymerase II C-terminal domain nonconsensus repeats in CTD stability and cell proliferation.

Biochemical characterization of the molecular chaperone Hsp90, which delivers precursor proteins to the chloroplast import receptor.

Comparison of different families of variant surface glycoproteins of T. brucei and their potential role in protease inactivation.

Preparation of EST sequencing reactions, sequence analysis, and genome assembly.

Major subject: Genetics – Minor subjects: Anthropology and Human Genetics, Cell Biology, Immunology, Neurobiology