Dr. Suliman Qadir Afridi

is looking for a new team member.

Bis 2024, Wissenschaftler/scientist Vaccine technology, Fraunhofer-Gesellschaft

Leipzig, Deutschland

Fähigkeiten und Kenntnisse

ELISA development
Downstreaming Processing
Molecular biology
T-cell assay
Protein expression and purification
Immunology
Intracellular cytokine profiling
primary cell isolation and culture
immune cells assay
neutralization assay
blocking assay
flow-cytometry
FACS sorting
structural analysis
live cell imaging
sequence analysis
molecular cloning
quantitative ELISA
western blotting
ultra-centrifugation
GLP (Good laboratory practise)
working in S2 laboratory
Inkscape
Chimera
Flowjo
Serial cloner
Mendeley
Endnote
MEGA X
Microsoft office tools
problem-solving ability
Critical thinking
Resilient and Adaptable
People Management
dynamic

Werdegang

Berufserfahrung von Suliman Qadir Afridi

  • 4 Jahre und 1 Monat, Feb. 2020 - Feb. 2024

    Wissenschaftler/scientist Vaccine technology

    Fraunhofer-Gesellschaft

    • Managing and leading projects involving diverse collaborators, students supervision • Conducting assay development and validation adhering to FDA and EU standards. • Evaluating filter performance with nebulized virus particles. • Contributing to upstream/downstream processing. • Developing SOPs and defining lifecycle validation for analytical test methods. • Proficient in RT-PCR, protein expression, western blotting, ELISA, and cell culture assays.

  • 2 Jahre und 4 Monate, Okt. 2018 - Jan. 2021

    Postdoctoral Wissenschaftler (Virology)

    Technische Universität München (Germany)

    • Project management and students supervision • Vaccine development • Assay development and validation • Cloning, protein expression and purification, ELISA, Chromatography, western blotting, PCR, RT PCR, Cell culture, FFU assay, neutralization assay, TCID50, molecular dynamics, molecular docking, paper writing, project designing,

  • 4 Jahre und 5 Monate, Okt. 2014 - Feb. 2019

    PhD Researcher – Medical life science and technology

    Technische Universität München (Germany)

    • To investigate evolutionary changes in norovirus of chronically infected patients, a quantitative ELISA was developed and used next-generation sequencing and structural modeling with molecular dynamics simulation • Production of recombinant norovirus capsid genes through molecular cloning to develop quantitative ELISA • Differential analysis of norovirus VP1 to study changes in structure of sequential samples from immunocompromised patients

  • 2 Monate, Juli 2018 - Aug. 2018

    Visiting Scientist

    Prof. Micheal Hougton (Nobel Prize laureate) Lab, University of Alberta, Canada

    • Application of novel therapeutic approaches to control and eradicate HCV infection • Live cell imaging using time lapse microscopy • Cell culture of HEK293 line

  • 1 Jahr und 5 Monate, Sep. 2012 - Jan. 2014

    Microbiologist

    Ideal Medical Laboratory and Research Centre Ltd, Lahore (Pakistan)

    • Identification of different pathogens using real time PCR, conventional PCR, quantitative PCR and Nested PCR, blood chemistry • Clinical chemistry, drug level determinations, endocrinology and HIV / hepatitis serology • Blood serology • Implementation of comprehensive legal and institute quality assurance systems

  • 3 Jahre und 2 Monate, Feb. 2010 - März 2013

    Senior Medical Technologist

    The Medical Laboratory Ltd, Lahore (Pakistan)

    • DNA extraction and genotyping of HCV and other pathogens • Hands-on experience of different analyzer i.e Architect, Modular, Elycsis analyzer • Detection of HIV, HBV, HCV, HDV, H.pylori and STDs via ELISA

  • 7 Monate, Dez. 2010 - Juni 2011

    Research Associate

    Quality Operation Laboratory, UVAS, Lahore (Pakistan)

    • DNA extraction and genotyping of HCV and other pathogens • Hands-on experience of different analyzer i.e Architect, Modular, Elycsis analyzer • Detection of HIV, HBV, HCV, HDV, H.pylori and STDs via ELISA

  • 3 Monate, Juni 2009 - Aug. 2009

    Research Assistant

    National Institute of Biotechnology and genetic engineering, (Pakistan)

    Preparation of media and culturing and purification of different novel fungal strains for bio-degradation of coal

Ausbildung von Suliman Qadir Afridi

  • 4 Jahre und 5 Monate, Okt. 2014 - Feb. 2019

    Molecular virology

    Technische Universität München (Germany)

    Molecular Medicine, Introduction to Virotherapy, Basic principles of light Microscope, Laboratory animal and comparative pathology, Zebrafish: Model organism to study development, neurobiology and behaviour, Immunobiology, Techniques in presenting scientific data, Good scientific practices

  • 2 Jahre und 1 Monat, Aug. 2010 - Aug. 2012

    Microbiology

    University of Veterinary and Animal Sciences, Lahore (Pakistan)

    Cell culture Techniques, Public health Microbiology, Advance Virology, Systemic Bacteriology, Molecular Medicine and Pharmaceuticals, Molecular Pathology

  • 4 Jahre und 1 Monat, Okt. 2005 - Okt. 2009

    Microbiology, molecular biology

    Kohat University of Science and Technology, Kohat, KPK (Pakistan)

    Microbiology, Virology, Fundamental of Immunology, Human Genetics, Medical Microbiology, Recombination DNA Technology, Cell Biology, Microbial Ecology, Soil Microbiology, Bioinformatics, Molecular Biology

Sprachen

  • Englisch

    Fließend

  • Pashto

    Muttersprache

  • Urdu

    Muttersprache

  • Hindi

    Fließend

  • punjabi

    Fließend

  • Deutsch

    Gut

Interessen

Reading
Fitness
swiming
traveling
Music
Hiking

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