madhura khare
Angestellt, Associate Scientist, Pieris Pharmaceuticals
Munich, Deutschland
Werdegang
Berufserfahrung von madhura khare
Bis heute 4 Jahre und 8 Monate, seit Okt. 2019
Associate Scientist
Pieris Pharmaceuticals
Bis heute 6 Jahre und 11 Monate, seit Juli 2017
online tutor
urbanpro
Online tutoring is the freelancing job. I teach high school and graduate students online in Biology, Biochemistry, bioinformatics, proteomics etc.
7 Monate, Nov. 2016 - Mai 2017
Research assistant
National Chemical Laboratory
• Amplification and Gene cloning of various H. Armigera Gut Trypsins into bacterial expression vectors like pET28a, pEGX etc. • Optimizing culture induction temperatures and Bulk expression and purification using chromatographic techniques. • Setup crystallization trials for purified protein. Gene cloning and expression, Protein Purification, Akta system: Gel filtration, Ion exchange, affinity and desalting column.
1 Jahr und 1 Monat, Aug. 2015 - Aug. 2016
Junior Research Fellow
National Center for Cell Science (NCCS)
• Worked on “Profiling and elucidating the role of Exosomes in inducing aggressiveness in normal cells”. • Assisted in optimization of isolation of exosomes from spent media using gradient ultracentrifugation and immuno-precipitation on dyna beads. Cell culture, Sucrose gradient ultracentrifugation, RNA Isolation and c-DNA synthesis, Immuno-precipation.
1 Jahr und 1 Monat, Juli 2014 - Juli 2015
Junior Research Fellow
Advanced Cancer Treatment, Research and Educational Cen.
• Record maintainance of Blood Sample (HNSCC) collected along with its meta-data. • Selected pathogenic mutations were modeled and simulated in-silico for 100ns out to see the related structural changes Gene cloning and expression, Protein Purification, Gel filtration column (S75, S200), LC MS/MS data analysis, Ammonium sulphate precipitation SQL, Biological databases and pathway analysis HTML, CSS and JavaScripts.
6 Monate, Jan. 2014 - Juni 2014
Project Trainee/Master’s thesis
National Institute of Research for Reproductive Health (NIRRH)
• Worked on “MD simulation studies of various antimicrobial peptides in TFE: Water environment” Gromacs, Unix/Linux operation, sequence analysis, remote-local server access
Ausbildung von madhura khare
2 Jahre und 3 Monate, Juni 2012 - Aug. 2014
Bioinformatics, Biotechnology
D. Y. Patil University
Biotechnology, Bioinformatics, Molecular Biology, Biochemistry
4 Jahre und 2 Monate, Juli 2007 - Aug. 2011
Biomedical Engineering
University of Mumbai
Human Anatomy, Physiology, transducers
Sprachen
Englisch
Fließend
Deutsch
Gut