Saradaprasan Muduli

is working from home. 🏡

Angestellt, Doctoral Researcher, RWTH Aachen University - Institute of Technical and Macromolecular Chemistry

Aachen, Deutschland

Fähigkeiten und Kenntnisse

Molecular Biology
Research
Engineering
Publication
Bacteria Cell Culture
Cell Culture Technology
Genetics
Cancer Research
Confocal Microscopy
Western Blotting
Genomics
Mammalian Cell Culture
Laboratory Skills
Biomedical Sciences
Data Analysis
Biochemistry
Communication skills
Leadership skills
Project management
Biotechnology
Polymerase chain reaction
ELISA
SDS-PAGE
qPCR
Molecular Cloning
Stem Cell Research
Cellular Differentiation
Disease modelling
Genetic Engineering
CRISPR/Cas9
Transfection
Cell Line Generation
MTT Assay
Live and Dead Assay
Plasmid preparation
Nucleic Acid Chemistry
Rolling Circle Transcription
Isothermal Titration Calorimetry
Mo-Flo Astrios
Flow Cytometry
Flourescent Microscope
RNA extraction
DNA isolation
Synthetic biology
In-vitro Transcription
In-vitro cleavage assay
Surveyor Assay
Zellbiologie
Forschung und Entwicklung
Entwurf

Werdegang

Berufserfahrung von Saradaprasan Muduli

  • Bis heute 6 Jahre und 1 Monat, seit Mai 2018

    Doctoral Researcher

    RWTH Aachen University - Institute of Technical and Macromolecular Chemistry

    Generated transgenic human cell lines for on-demand mRNA expression by engineering recombinant DNA sequences through molecular cloning. Devised techniques for drug localisation in cell nuclei utilizing modified antibiotics. • Designed, improved and developed chemically inducible gene expression system in HEK293T and cervical cancer cells. • Identified toxicities of aminoglycosides in kidney cells and cervical cancer cells.

  • 1 Jahr und 3 Monate, Feb. 2017 - Apr. 2018

    Project Manager as Graduate Research Assistant

    National Central University - Department of Life Science

    Studied large data sets applying bioinformatics approach to uncover and establish genetic linkages of human heart diseases. Used ELISA and immunostaining to evaluate protein production for characterising the in-vitro disease model. • Examined CRISPR/Cas9 mediated gene knock-out and engineered cell-based cardiomyopathy models

  • 2 Jahre und 5 Monate, Sep. 2014 - Jan. 2017

    Cell Culture Process Engineering as GRaduate Research Assistant

    National Central University

    Applied genetic alterations through sendai virus and reprogrammed adult stem cells into pluripotent stem cells. Engineered biopolymers for enhanced stem cell isolation from amniotic fluid resulting in two publications. • Published four articles on bone formation and bone-specific mRNA expression in adult stem cells on elastic biomaterial.

Ausbildung von Saradaprasan Muduli

  • 3 Jahre, Mai 2018 - Apr. 2021

    Chemistry

    RWTH Aachen University

    Genetic Engineering

  • 1 Jahr und 11 Monate, Sep. 2014 - Juli 2016

    Chemical and Materials Engineering

    National Central University

    Thesis: Proliferation & osteogenic differentiation of hAFSCs cultured on biomaterials having nano segments and optimal elasticity

  • 2 Jahre und 11 Monate, Juni 2011 - Apr. 2014

    Chemistry

    Ravenshaw University

Sprachen

  • Englisch

    Muttersprache

  • Deutsch

    Grundlagen

  • Chinesisch

    Grundlagen

Interessen

Gymnastics
Road cycling
Painting
Astrophysics
Crossfit
Cricket
Book Collection
Computer Hardware
Cooking for friends

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